Methods in gut microbial ecology for ruminants pdf

The micro-organisms in the digestive tracts of ruminant livestock have a profound influence on the conversion of feed into end products, which can impact on the animal and the environment.

As the livestock sector grows particularly in developing countries, there will be an increasing need to understand these processes for better management and use of both feed and other natural resources that underpin the development of sustainable feeding systems.

Newgene-based technologies can nowbe employed to examine microbial diversity through the use of small sub-unit ribosomal DNA analysis e. These technologies have the potential to revolutionize the understanding of rumen function and will overcome the limitations of classical-based techniques, including isolation and taxonomic identification of strains important to efficient rumen function and better understanding of the roles of micro-organisms in relation to achieving high productivity and decreasing environmental pollutants.

It gives a comprehensive up-to-date account of the methodologies and the protocols for conventional and modern molecular techniques that are currently in use for studying the gut microbial ecology of ruminants. Each chapter has been contributed by experts in the field. The techniques and procedures described are also relevant and adaptable to other gastrointestinal ecosystems and the microbiology of anaerobic environments in general.

The future of ruminant gut microbiology research is dependent upon the adoption of these molecular-based research technologies, and the challenge at present is the use of these technologies to improve ruminant production and decrease environment pollutants through a better understanding of microbial function and ecology.

It is hoped that this book will equip the readers better in order to meet this unprecedented challenge. Methods in Gut Microbial Ecology for Ruminants. VetBooks August 6, Denaturing gradient gel electrophoresis. Svetlana A. Kocherginskaya, Isaac K. Cann, Roderick I. Bacteriophage populations. Anaerobic fungal populations. Jayne L. Brookman, Matthew J. Denman, Makoto Mitsumori, Christopher S. Wright, Kiyoshi Tajima, Rustam I. Northern blot analysis to investigate the abundance of microorganisms.

Whole cell probing with fluorescently labelled probes for in situ analysis of microbial populations. Metagenomic analysis of the microbiomes in ruminants and other herbivores. Mark Morrison, Sarah E. Adams, Karen E. Nelson, Graeme T. Back Matter Pages About this book Introduction Asaresultofvarioushumanactivities,suchasincreaseinhumanpopulation,decrease in arable land due to soil degradation, urbanization, industrialization and associated increase in the demand for livestock products, dramatic changes are occurring in the global ruminant livestock sector.

These changes includeshift inthesize of regional livestock populations and in the types of management and feeding systems under which ruminant livestock are held, and increased demand of a wider range of quality attributes from animal agriculture, not just of the products themselves but also of the methods used in their production. The livestock sector will need to respond to newchallengesofincreasinglivestockproductivitywhileprotectingenvironmentand human health and conservingbiodiversity and natural resources.

The micro-organisms in the digestive tracts of ruminant livestock have a profound in? As the livestock sector grows particularly in developing countries, there will be an increasing need to understand these processes for b- ter management and use ofbothfeed and other natural resources that underpinthe development of sustainable feeding systems.

Editors and affiliations. Harinder P. Makkar 1 Christopher S. McSweeney 2 1.